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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
MODIFICATION ENZYMES
BamH I Methylase

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

BamH I Methylase
EBR-3004
200 unit (5 unit/㎕)
80,000
DESCRIPTION
The BamH I Methylase recognizes 5´--GGATCC--3´ sequences and methylates internal cytosine (C⁴) residues.

Source
- Recombinant gene from Bacillus amyloliquefaciens H.

Applications
- Blocking restriction endonuclease cleavage restricted by BamH I methylation.

Reaction Conditions
- 1x Methylase reaction buffer in the presence of 160 μM S-adenosylmethionine (SAM). Incubate at 37℃.

Concentration & Storage Condition
- Store at -20℃. 5 unit/㎕ in 50 mM Tris-HCl, pH 7.5, 1 mM DTT, 10 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.

Unit Definition
- One unit is defined as the amount of enzyme required to fully protect against BamH I cleavage of 1 ㎍ of λ DNA in 1 hr at 37℃.

Heat Inactivation Condition
- 65℃ for 20 min.

QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

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