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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
MODIFICATION ENZYMES
Bst DNA Polymerase, exo- (15,000 unit)

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Bst DNA Polymerase, exo- (15,000 unit)
EBT-1702
15,000 units (60 unit/㎕)
320,000
DESCRIPTION
Bst DNA Polymerase is a recombinant DNA polymerase from Bacillus stearothermophilus. Bst DNA Polymerase is purified from an E.coli strain carrying a plasmid with the cloned gene encoding large fragment of DNA polymerase. It contains the 5' to 3´ polymerase activity, but lacks 5' to 3´ exonuclease activity. For this reason, Bst DNA Polymerase can be effectively used in chain displacement reaction at high temperature, 65°C.

Storage Buffer
- 60 unit/㎕ in 10 mM Tris-HCl, pH8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and 50% Glycerol.

10x Reaction Buffer w/ MgCl₂
- 200 mM Tris-HCl, pH8.8, 100 mM (NH₄)₂SO₄, 100 mM KCl, 20 mM MgSO₄, 1% Triton X-100.

Unit Definition
- One unit of enzyme incorporates 10 nmole of dNTP into acid insoluble material in 30 min at 65°C.

Storage Condition
- Store at -20°C.

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