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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
RESTRICTION ENZYMES
Dra III

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Dra III
EBR-1051
2,500 unit (10 unit/㎕)
70,000
DESCRIPTION
Source
- Deinococcus radiophilus.

Reaction Conditions
- Buffer 3 + BSA. 50 mM Tris-HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 37℃.

Concentration & Storage
- Store at -20℃. 10 unit/㎕ in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.

Heat Inactivation
- Condition 65℃ for 20 min. Enzyme Dilution Dilute with Storage Buffer.

QC Tests
- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

Methylase Sensitivity
- Impaired by overlapping CpG methylation.

Note
- Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or large excess of enzyme may result in star activity.

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