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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
GLYCOSIDASES
Endo H (Endoglycosidase H)

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Endo H (Endoglycosidase H)
EBG-1001
50,000 unit (500 unit/㎕)
300,000
DESCRIPTION
Endo H (Endoglycosidase H) is a recombinant glycosidase expressed and purified from E.coli which carries the Streptomyces plicatus gene.

Endo H cleaves between the N-acetylglucosamine residues of the chitobiose core of N-linked glycans, leaving one N-acetyl glucosamine residue attached to the asparagine.

Endo H has a molecular weight of ~31.1 kDa.

The workable pH range is between 5.0 to 6.0, with the optimal pH at 5.5.

Substrate Specificity
- High mannose oligosaccharides
- Hybrid N-linked oligosaccharides

Product Component
- Endo H : 100 ㎕ (100 unit/㎕)
- 10x Reaction Buffer : 500 mM sodium citrate, pH 5.5.

Unit Definition
- One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 ㎍ of denatured RNase B for 1 hr at 37°C in a total reaction volume of 10 ㎕.

Specific Activity
- 500 unit/㎕, 400-500 unit/㎍

Storage Buffer
- 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM EDTA, 50% glycerol.

Endo H Reaction Condition
- 10 ㎍ of RNase B is denatured with 0.5% SDS, 1% β-mercaptoethanol at 100°C for 10 min. After the addition of Reaction Buffer to final 1x, Endo H are added and the reaction mix is incubated for 1 hr at 37°C. Separation of reaction products is analyzed by SDS-PAGE (cleaved RNase B migrates faster). The activity of Endo H is not affected by addition of 0.5% SDS. For a native glycoprotein, 5-fold more enzyme and longer incubation time will be required.

Storage Temperature
- Store at -20℃.

QC Tests
- Activity, SDS-PAGE purity, performance tests.

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