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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
MODIFICATION ENZYMES
Exonuclease III (E.coli)

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Exonuclease III (E.coli)
EBT-3025
10,000 unit (100 unit/㎕)
80,000
DESCRIPTION
Exonuclease III catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from
the termini of DNA fragments to construct a series of deletion mutants.
Exonuclease III is purified from a recombinant E.coli strain.

Concentration & Storage Condition
- 100 unit/㎕. Store at -20℃.

Storage Buffer
- 20 mM Tris-HCl, pH 7.5, 1 mM DTT, 100 mM KCl, 50% glycerol.

10x Reaction Buffer
- 660 mM Tris-HCl, pH 8.0, 6.6 mM MgCl₂.

Unit Definition
- One unit is defined as the amount of enzyme required to produce 1 nmole of acid-soluble nucleotides from double-stranded DNA within 30 min at 37°C.

QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

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