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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
MODIFICATION ENZYMES
Hae III Methylase

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Hae III Methylase
EBR-3006
1,000 unit (5 unit/㎕)
80,000
DESCRIPTION
The Hae III methylase recognizes 5´--GGCC--3´ sequences and methylates internal cytosine (C⁵) residues.

Source
- Recombinant gene from Haemophilus aegyptius.

Applications
- Blocking restriction endonuclease cleavage restricted by Hae III methylation.

Reaction Conditions
- 1x Methylase reaction buffer in the presence of 160 μM S-adenosylmethionine (SAM). Incubate at 37℃.

Concentration & Storage Condition
- Store at -20℃. 10 unit/㎕ in 50 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 10 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.

Unit Definition
- One unit is defined as the amount of enzyme required to fully protect against Hae III cleavage of 1 ㎍ of λ DNA in 1 hr at 37℃.

Heat Inactivation Condition
- 65℃ for 20 min.

QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

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