RESTRICTION ENZYMES
Mbo II
PRODUCTS NAME CAT # QUANTITY PRICE (W) MORE INFORMATION
Mbo II
EBR-1087
250 unit (5 unit/㎕)
80,000
DESCRIPTION
Source
- Moraxella bovis.
Reaction Conditions
- Buffer 4. 20 mM Tris-acetate, pH 7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Incubate at 37℃.
Concentration & Storage Condition
- Store at -20℃. 5 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol, 0.15% Triton X-100.
Heat Inactivation Condition
- 65℃ for 20 min.
Enzyme Dilution
- Dilute with Storage Buffer.
QC Tests
- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.
Methylase Sensitivity
- Not sensitive to dcm or CpG methylation. Blocked by overlapping dam methylation.