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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
RESTRICTION ENZYMES
Nde I

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Nde I
EBR-1099
5,000 unit (10 unit/㎕)
70,000
DESCRIPTION
Source
- Neisseria denitrificans.

Reaction Conditions
- Buffer 4. 20 mM Tris-acetate, pH 7.9, 50 mM potassium acetate, 10 mM magnesium acetate, 1 mM DTT. Incubate at 37℃.

Concentration & Storage Condition
- Store at -20℃. 10 unit/㎕ in 10 mM Tris-HCl, pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol.

- Heat Inactivation Condition
65℃ for 20 min.

Enzyme Dilution
- Dilute with Storage Buffer.

QC Tests
- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

Methylase Sensitivity
- Not sensitive to dam, dcm or CpG methylation.

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