CALL US

Tel: 042-581-8448 | Fax: 042-581-8449

EMAIL US
OPENING HOURS

Mon - Fri: 9am - 6pm

OVER 20 YEARS EXPERIENCE

- Since 1999

OUR SERVICES

- Recombinant Antibody Screening 

- Recombinant Antibody Purification

VISIT US

More than 400 specialized products for molecular biology and protein biochemistry

  • Facebook Social Icon
  • Twitter Social Icon
  • Google+ Social Icon
  • YouTube Social  Icon
  • Pinterest Social Icon
  • Instagram Social Icon

© 2018 by ELPISBIOTECH. INC

MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
RESTRICTION ENZYMES
Not I

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Not I
EBR-1103
500 unit (10 unit/㎕)
70,000
DESCRIPTION
Source
- Nocardia otitidis-caviarum.

Reaction Conditions
- Buffer 3 + BSA. 50 mM Tris-HCl, pH 7.9, 100 mM NaCl, 10 mM MgCl₂, 1 mM DTT. Supplement with 100 ㎍/ml BSA. Incubate at 37℃.

Concentration & Storage Condition
- Store at -20℃. 10 unit/㎕ in 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 ㎍/ml BSA, 50% (v/v) glycerol, 0.15% Triton X-100.

Heat Inactivation Condition
- 65℃ for 20 min.

Enzyme Dilution
- Dilute with Storage Buffer.

QC Tests
- Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

Methylase Sensitivity
- Blocked by CpG methylation (cleavage of mammalian genomic DNA is blocked).

Note
- Supercoiled plasmids may required up to 5 folds more Not I for complete digestion than linear DNAs.

1/1