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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
RT-PCR KITS
RT Prime Kit

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

RT Prime Kit
EBT-1520
1 kit (50 RT)
140,000
DESCRIPTION
RT Prime Kit is designed for the reverse transcription (RT) from either total RNA or polyA⁺ RNA.
RT Prime Kit provides reagents sufficient for 50 RT reactions.

The kit contains M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase RNase H⁻. Both random hexamers and oligo d(T)15 are included, allowing cDNA synthesis from any RNA source.

The kit is optimized for 20 ㎕ RT reaction and each cDNA synthesis may be divided and used in up to 20 separate PCR reactions.

The kit contains control total RNA and primer pair for a positive control experiment.

Applications
- First strand cDNA synthesis from total RNA or poly A⁺ RNA for library construction and RT-PCR.

QC tests
- Performance tests.

Storage Condition
- Store at -20℃.

Kit Component
- M-MLV Reverse Transcriptase, RNase H⁻ : 10,000 unit (200 unit/㎕)
- 5x Reverse Transcription Buffer : 500 ㎕
- 10 mM dNTP : 500 ㎕
- Random Hexamer : 50 ㎕(100 pmol/㎕)
- Oligo d(T)15 : 50 ㎕ (100 pmol/㎕)
- Recombinant RNase Inhibitor : 2,500 unit (50 unit/㎕)
- Total RNA, Human 293 cell : 10 ㎕ (1 ㎍/㎕)
- Control Primer Pair Mix, β-actin : 20 ㎕ (10 pmol/㎕)
- RNase-Free Water : 1 ml

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