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Rapid-Con Protein Concentration Kit

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Rapid-Con Protein Concentration Kit
EBE-1012
1 Kit
100,000
DESCRIPTION
Rapid-Con Protein Concentration Kit enables the user to easily perform the following routine procedures;
1) rapid concentration of diluted proteins and 2) removal of various contaminating substances in protein samples, such as guanidine, urea, detergents, nucleotides, lipids, organic solvents, or other common salts.

Appropriate concentration of protein and prior removal of contaminating substances from protein samples are critical factors for subsequent analysis such as SDS-PAGE.

The Rapid-Con Protein Concentration Kit contains modified silicates support which can bind proteins in an aqueous phase.

The bound proteins can be easily liberated by boiling in the presence of PAGE sample loading buffer, by addition of 1% SDS/5 mM EDTA, or raising buffer pH up to 10.

- Diluted protein concentration
- Protein concentration in cell culture medium (serum-free)
- Removal of contaminated materials from protein like lipids, saccharides, and nucleotides
- Removal of contaminated salts
- Removal of contaminated dyes

Kit Components
- Protein concentration bead : 0.5 ml
- 2x Binding buffer : 30 ml
- Washing buffer : 60 ml
- Elution buffer : 1 ml (50 mM Tris-HCl, pH8.0, 5 mM EDTA, and 1% SDS)

Cautions
- This kit can not be used for recovery and concentration of native and functional proteins. The protocol provides only for denatured proteins.
- Maximum binding capacity : <14 mg/ml of BSA in standard condition.
- The entire kit can be stored at room temperature. This product is guaranteed for one year from the date of purchase when handled and stored properly.

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