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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
SITE-SPECIFIC ENDOPROTEASES
TEV Protease, 6xHis (500 ㎕)

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

TEV Protease, 6xHis (500 ㎕)
EBF-1010
10,000 unit (20 unit/㎕)
800,000
DESCRIPTION
TEV protease is a site-specific endoprotease that cleaves after glutamine at its cleavage site Glu-Asn-Leu-Tyr-Phe-Gln-/-Gly.

Recombinant TEV protease is purified from E.coli containing a clone of the Tobacco Etch Virus gene.

Since TEV protease contains 6x His sequence (EBF-1009 and 1010) or 6x His-GST (EBF-1011 and 1012), they can be easily removed by a metal affinity chromatography or GSH affinity chromatography after the cleavage reaction.

The recombinant 6x His tagged TEV protease is 28.6 kDa, and 6x His-GST tagged TEV protease is 58.1 kDa in size.

Substrate Specificity
- -Glu-Asn-Leu-Tyr-Phe-Gln - / - Gly-

Product Component
- TEV Protease : 500 ㎕ (500 ㎍, 10,000 units)
- Control protein : 30 ㎍ (1 ㎍/㎕), produced 45 kDa and 48 kDa by TEV protease

Unit Definition
- One unit of TEV cleaves ≥95% of 3 μg control substrate for 1 hr at 30°C.

Specific Activity
- TEV Protease (6x His Tag) : 20 unit/㎍ protein

Storage Buffer
- 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 5 mM DTT, 50% glycerol, 0.1% Triton X-100.

TEV Reaction Condition
- 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, 1 mM DTT, 150 mM NaCl.
- caution: reaction buffer is not included in the product

Storage Temperature
- Store at -20℃.

QC Tests
- Activity, SDS-PAGE purity, performance tests.

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