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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
MODIFICATION ENZYMES
Thermostable Pyrophosphatase

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Thermostable Pyrophosphatase
EBT-3039
50 ㎍ (0.5 ㎍/㎕)
100,000
DESCRIPTION
An E. coli strain carrying a plasmid encoding pyrophosphatase from the extreme thermophile Thermococcus litoralis. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.

Concentration & Storage Condition
- 0.5 ㎍/㎕. Store at -20℃.

Storage Buffer
- 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0 at 25°C

Unit Definition
- One unit is the amount of enzyme that will generate 1 μmol of phosphate per min from inorganic pyrophosphate under standard reaction conditions (a 10 min reaction at 75°C in 50 mM Tricine, pH 8.5 , 1 mM MgCl₂, 0.32 mM PPi, reaction volume of 0.5 ml).

QC Tests
- Activity, SDS-PAGE purity, performance tests

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