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MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
MODIFICATION ENZYMES
Uracil DNA Glycosylase (UDG)

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

Uracil DNA Glycosylase (UDG)
EBT-3071
1,000 unit (2 unit/㎕)
150,000
DESCRIPTION
Uracil-DNA Glycosylase, also known as UDG or UNG, removes free uracil residues from uracil-containing DNA (single- and double-stranded) without destroying the sugar-phosphodiester backbone.UDG effectively prevents the subsequent use of UDG-treated DNA as a hybridization target or as a template for DNA polymerases.

Treatment of 0.1 ㎍ of uracil-containing DNA with 1 unit UDG for 10 min at 37°C can not be used as a template for next round of PCR. UDG is active over a broad range of pH and heat inactivated > 95% by incubation at 95°C for 10 min.

Uracil-DNA Glycosylase gene is obtained from E.coli strain and recombinant enzyme is purified.

Concentration & Storage Condition
- 2 unit/㎕, Store at -20℃.

10x Reaction Buffer
- 200 mM Tris-HCl, pH7.9, 10 mM DTT, 10 mM EDTA.

Storage Buffer
- 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA and 50% glycerol.

QC Tests
- Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

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