CALL US

Tel: 042-581-8448 | Fax: 042-581-8449

EMAIL US
OPENING HOURS

Mon - Fri: 9am - 6pm

OVER 20 YEARS EXPERIENCE

- Since 1999

OUR SERVICES

- Recombinant Antibody Screening 

- Recombinant Antibody Purification

VISIT US

More than 400 specialized products for molecular biology and protein biochemistry

  • Facebook Social Icon
  • Twitter Social Icon
  • Google+ Social Icon
  • YouTube Social  Icon
  • Pinterest Social Icon
  • Instagram Social Icon

© 2018 by ELPISBIOTECH. INC

MOLECULAR BIOLOGY
PROTEIN BIOCHEMISTRY
DNA CLONING KITS
pLPS-B Blunt Topo Vector, 25 rxn

    PRODUCTS NAME                                                          CAT #              QUANTITY                      PRICE (W)    MORE INFORMATION  

pLPS-B Blunt Topo Vector, 25 rxn
EBK-1001
1 Kit (25 rxn)
200,000
DESCRIPTION
The pLPS-B blunt topo vector is a high efficient and very convenient tool for a direct cloning of PCR products. The type I topoisomerase from Vaccinia virus can cut and rejoin the DNA fragment without the action of DNA ligase.

Topoisomerase I premixed in the vector solution rejoins the input DNA fragments to vector ends within 5 min at 37℃. There is no need to consider whether insert DNA has been phosphorylated. Furthermore, there is no need to use IPTG/X-gal in the transformation process due to its high ligation and cloning efficiency (up to 90%). This means you can obtain 9 real clones from 10 randomly picked transformed colonies.

The pLPS-B blunt vector is suitable for the cloning of blunt ended DNA fragments. If your DNA has 3'-A tails, you should remove A tails by appropriate methods before start.

In general, we recommend the use of high fidelity DNA polymerases, like Pfu, Pfu plus, PyroMax, to produce the blunt ended PCR products. By using pLPS-B vector system, up to 10 kbp sized DNA fragments can be easily cloned without the remarkable loss of efficiency.

The pLPS-B blunt vector is provided in 50% buffered glycerol. Simply mix 1 ㎕ of vector solution with 1 ㎕ of 5x reaction buffer and your DNA to final 5 ㎕ volume, and incubate for 5 min at 37℃. Then you are already ready to transform E.coli. In the next day, you can get the clone in plasmid.

Kit Component : EBK-1001
- 25 ㎕ pLPS-B Blunt Vector
- 50 ㎕ 5x Topo Reaction Buffer
- 5 ㎕ Control Insert DNA (blunt ended)

QC tests
- Performance tests.

Storage Condition
- Store at -20℃.

Vector Sequence

1/1