What is a recombinant antibody?
-
Recombinant antibody is the smallest functional unit of antibody, containing only variable region of immunoglobulin that is sufficient for antigen recognition
-
Recombinant means antibodies are produced from antibody gene libraries and expressed in E.coli using a recombinant technology
What kinds of recombinant antibodies are available?
How to make recombinant antibodies?
-
Recombinant antibodies are selected from antibody gene libraries by so called biopanning process in vitro
-
Then, antibody clones showing the best characteristics are over-expressed and purified using an E.coli expression system
Main features of recombinant antibody?
-
No animal used in antibody screening and production process unlike poly or monoclonal antibody production
-
Minimized batch-to-batch variations in the functional aspects
-
Small size of recombinant antibodies may have advantage of improving the resolution in the filed of imaging study :
full IgG (~150 kDa) vs. scFv (~25 kDa) and sdAb (~15 kDa)
-
Recombinant antibody can be used in various applications : ELISA, WB, IHC, IF, IP, and so on
-
Recombinant antibody is a prospectiv tool in the fields of immunotherapy and immunodiagnosis currently
For more details, click here