What is a recombinant antibody?
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Recombinant antibody is the smallest functional unit of antibody, containing only variable region of immunoglobulin that is sufficient for antigen recognition
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Recombinant means antibodies are produced from antibody gene libraries and expressed in E.coli using a recombinant technology
What kinds of recombinant antibodies are available?
How to make recombinant antibodies?
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Recombinant antibodies are selected from antibody gene libraries by so called biopanning process in vitro
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Then, antibody clones showing the best characteristics are over-expressed and purified using an E.coli expression system
Main features of recombinant antibody?
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No animal used in antibody screening and production process unlike poly or monoclonal antibody production
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Minimized batch-to-batch variations in the functional aspects
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Small size of recombinant antibodies may have advantage of improving the resolution in the filed of imaging study :
full IgG (~150 kDa) vs. scFv (~25 kDa) and sdAb (~15 kDa)
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Recombinant antibody can be used in various applications : ELISA, WB, IHC, IF, IP, and so on
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Recombinant antibody is a prospectiv tool in the fields of immunotherapy and immunodiagnosis currently
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