재조합 항체 스크리닝의 대표브랜드
What is a recombinant antibody?
Recombinant antibody is the smallest functional unit of antibody, containing only variable region of immunoglobulin that is sufficient for antigen recognition
Recombinant means antibodies are produced from antibody gene libraries and expressed in E.coli using a recombinant technology
What kinds of recombinant antibodies are available?
We have two types of recombinant antibody ; single domain antibody (sdAb, camel's) and single chain antibody (scFv, human)
How to make recombinant antibodies?
Recombinant antibodies are selected from antibody gene libraries by so called biopanning process in vitro
Then, antibody clones showing the best characteristics are over-expressed and purified using an E.coli expression system
Main features of recombinant antibody?
No animal used in antibody screening and production process unlike poly or monoclonal antibody production
Minimized batch-to-batch variations in the functional aspects
Small size of recombinant antibodies may have advantage of improving the resolution in the filed of imaging study :
full IgG (~150 kDa) vs. scFv (~25 kDa) and sdAb (~15 kDa)
Recombinant antibody can be used in various applications : ELISA, WB, IHC, IF, IP, and so on
Recombinant antibody is a prospectiv tool in the fields of immunotherapy and immunodiagnosis currently
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