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재조합 항체 스크리닝의 대표브랜드 

RECOMBINANT ANTIBODIES

What is a recombinant antibody?

  • Recombinant antibody is the smallest functional unit of antibody, containing only variable region of immunoglobulin that is sufficient for antigen recognition

  • Recombinant means antibodies are produced from antibody gene libraries and expressed in E.coli using a recombinant technology

What kinds of recombinant antibodies are available?

  • We have two types of recombinant antibody ; single domain antibody (sdAb, camel's) and single chain antibody (scFv, human)

How to make recombinant antibodies?

  • Recombinant antibodies are selected from   antibody gene libraries by so called biopanning process in vitro

  • Then, antibody clones showing the best characteristics are over-expressed and purified using an E.coli expression system

 

Main features of recombinant antibody?

  • No animal used in antibody screening and production process unlike poly or monoclonal antibody production

  • Minimized batch-to-batch variations in the functional aspects

  • Small size of recombinant antibodies may have advantage of improving the resolution in the filed of imaging study : 

           full IgG (~150 kDa) vs. scFv (~25 kDa) and sdAb (~15 kDa)

  • Recombinant antibody can be used in various applications : ELISA, WB, IHC, IF, IP, and so on

  • Recombinant antibody is a prospectiv tool in the fields of immunotherapy and immunodiagnosis currently

 For more details, click here