Description

Bst DNA Polymerase exo- is a recombinant DNA polymerase from Bacillus stearothermophilus. Bst DNA Polymerase is purified from an E.coli strain carrying a plasmid with the cloned gene encoding large fragment of DNA polymerase. It contains the 5´-> 3´ polymerase activity, but lacks 5´-> 3´ exonuclease activity. For this reason, Bst DNA Polymerase can be effectively used in chain displacement reaction at high temperature, 65°C.

Applications
- Isothermal amplification (LAMP)
- Applications requiring strand-displacement DNA synthesis
- DNA sequencing through high GC regions
- Rapid sequencing from nanogram amounts of DNA template

Storage Buffer
120 unit/㎕ in 10 mM Tris-HCl, pH8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and 50% Glycerol.

10x Reaction Buffer w/ MgSO4
200 mM Tris-HCl, pH8.8, 100 mM (NH4)2SO4, 700 mM KCl, 30 mM MgSO4, 0.1% Tween20.

Unit Definition
One unit of enzyme incorporates 10 nmole of dNTP into acid insoluble material in 30 min at 65°C.

QC Tests
Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

Storage Condition

Store at -20℃.