EzPCR™ BASIC 2X PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template. EzPCR™ BASIC 2X PCR Master Mix contains rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus. rTaq DNA polymerase has a 5’ to 3’ exonuclease activity and generates a 3’dA overhang. EzPCR™ BASIC 2X PCR Master Mix is recommended for use in conventional PCR process.

As Master Mix is supplied as a 2X concentration format, users should adjust a final reaction to 1X (if final reaction volume is 20 ㎕, 10 ㎕ of 2X Master Mix should be added).

One unit of rTaq DNA polymerase is contained in 10 ㎕ of 2X Master Mix.

QC tests

Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.

Storage Condition

Store at -20 ℃.

EzPCR™ BASIC 2X PCR Master Mix is stable for at least 1years at recommended storage condition

 

Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows

 

EzPCR™ BASIC 2X Master Mix	10 ㎕
Primer (10 pmol/㎕)	each 0.5 ㎕
Template DNA	0.1 - 50 ng
PCR grade distilled water	- ㎕
Add distilled water to make 20 ㎕ final volume.

 

2. Set PCR cycling as follows

 

Initial denature at 95 ℃ : 3 min
		< 1 kbp	> 1 kbp
Denature	95 ℃	10 - 30 sec	30 sec
Anneal	Tm ℃	10 - 20 sec	30 sec
Extend	72 ℃	10 - 30 sec	1 min / Kbp

 

   * Set 25-35 PCR cycles for effective amplification.