TEV protease is a site-specific endoprotease that cleaves after glutamine at its cleavage site Glu-Asn-Leu-Tyr-Phe-Gln-/-Gly.
Recombinant TEV protease is purified from E.coli containing a clone of the Tobacco Etch Virus gene.Since TEV protease contains 6x His sequence (EBF-1009 and 1010) or 6x His-GST (EBF-1011 and 1012), they can be easily removed by a metal affinity chromatography or GSH affinity chromatography after the cleavage reaction.The recombinant 6x His tagged TEV protease is 28.6 kDa, and 6x His-GST tagged TEV protease is 58.1 kDa in size.Substrate Specificity- Glu-Asn-Leu-Tyr-Phe-Gln - / - Gly-Product Component- TEV Protease : 100 ㎕ (100 ㎍, 2,000 units)Unit Definition- One unit of TEV cleaves ≥95% of 3 μg control substrate for 1 hr at 30°C.Specific Activity- TEV Protease (6x His Tag) : 20 unit/㎍ proteinStorage Buffer- 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 5 mM DTT, 50% glycerol, 0.1% Triton X-100.TEV Reaction Condition- 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, 1 mM DTT, 150 mM NaCl.- caution: reaction buffer is not included in the productStorage Temperature
QC Tests- Activity, SDS-PAGE purity, performance tests.
