Antibody Format : sdAb

 

Fusion Protein : GB1

 

Antigen : E.coli ATP-dependent DNA helicase RecQ

 

UniProtKB : P15043

 

Species : E.coli

 

Species Cross-reactivity :

 

Remarks :​

 

Applications : Dilution

 

  ● ELISA

 

  ● WB  _

 

  ● IF  _

 

Description :

 

  ● Antigen Preparation : The 68.3 kDa full length protein was expressed in E.coli and purified by his affinity purification.

 

  ● Antigen Amino Acid Sequence : 

AQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

 

​Abbrevications and cautions :

 

  ● scFv : human single chain antibody (naive sequences)

 

  ● sdAb : camel single domain antibody (fully synthetic sequences)

 

  ● GB1 fusion : IgG binding domain of streptococcal protein G

 

  ● IgG Fc fusion : human, mouse or rabbit IgG Fc (contains hinge, CH1, CH2 domain)

 

  ● Applications : yes/no or _ (not determined)